Maram's Scientific Chronicles

So excited and proud to share that my work regarding the role of ubiquitin and the proteasome in DNA-protein crosslink repair has finally been published in Nucleic Acids Research! For those that don’t feel like reading the whole paper (please click on the link anyway!) see the findings that I am most excited about below!

In this study I generate a DNA damage substrate in vitro, transfect it into mammalian cells, and use various q-PCR based assays to show that:

1. A single type of DNA-protein crosslink (DPC) can undergo repair through multiple pathways. 
2. Different DPC repair pathways are mediated by different polyubiquitination signals on the DNA-crosslinked protein (K48 and K63). 
3. In a replication and transcription-independent context, DPC repair by NER, but not HR is proteasome dependent.

In this paper I also propose a novel model describing the orchestration of DPC repair in mammalian cells. I’m extremely excited about the future insights that can be made about DPC repair through further testing and exploration of this model!

See article here! Model below.